rabbit monoclonal anti nrf2 d1z9c  (Cell Signaling Technology Inc)

Journal: Cell Death Discovery

Article Title: Targeted temperature management alleviates post-resuscitation myocardial dysfunction by inhibiting ferroptosis

doi: 10.1038/s41420-025-02356-5

Figure Lengend Snippet: A Western blot of total Nrf2, cytoplasmic Nrf2, and nuclear Nrf2 proteins in myocardial tissues. B – D Quantification of total Nrf2, cytoplasmic Nrf2, and nuclear Nrf2 protein levels in myocardial tissues of each treatment group. ( n = 6) E Western blot of total Nrf2, cytoplasmic Nrf2, and nuclear Nrf2 proteins in myocardial tissues in cells. F – H Quantification of total Nrf2, cytoplasmic Nrf2, and nuclear Nrf2 protein levels in cells. ( n = 6) I Immunofluorescence staining of Nrf2 in different treatment groups (DAPI staining shows nuclei). Scale bar:20 μm. J Quantification of Nrf2 immunofluorescence intensity in different treatment groups. ( n = 6) K Immunoblot analysis of FPN1, FTH1, 4-HNE,Nuclear Nrf2 and NCOA4 under hypoxia/reoxygenation (H/R) conditions with or without Nrf2 activation. β-actin and Histone H3 are used as loading controls. L – P Quantification of FPN1, 4-HNE,NCOA4,FTH1 and nuclear Nrf2 protein levels after H/R treatment and Nrf2 activator treatment. ( n = 6) Q Oxidative stress markers:MDA levels, reflecting oxidative stress levels under hypoxia/reoxygenation (H/R) conditions with or without Nrf2 activation. R Fluorescence microscopy images of Fe2+ under hypoxia/reoxygenation (H/R) conditions with or without Nrf2 activation. S Relative fluorescence intensity of Fe 2+ . Measurement data were presented as mean ± SD. Statistical significance is indicated as follows: * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Membranes were then incubated with antibodies against GPX4 (1:1000, rabbit, ab125066,Abcam), 4-HNE(1:1000, rabbit, ab46545, Abcam), NCOA4 (1:1000, rabbit, ab314553, Abcam), FTH1 (1:1000, rabbit, GeneTex, GTX101733), Nrf2 (1:1000, rabbit, 60004–1-Ig Proteintech), β-actin (1:1000, rabbit, 81115-1-RR, Proteintech), Histone H3(1:1000, 17168-1-AP, Proteintech) overnight at 4 °C.

Techniques: Western Blot, Immunofluorescence, Staining, Activation Assay, Fluorescence, Microscopy